{"id":994,"date":"2022-08-02T00:23:12","date_gmt":"2022-08-02T07:23:12","guid":{"rendered":"https:\/\/leadinglifetechnologies.com\/?page_id=994"},"modified":"2025-09-11T12:26:29","modified_gmt":"2025-09-11T19:26:29","slug":"why-focaltuning-not-elisa","status":"publish","type":"page","link":"https:\/\/leadinglifetechnologies.com\/?page_id=994","title":{"rendered":"Why FocalTuning\u2122 a Superior Immunoassay Platform?"},"content":{"rendered":"\n

The FocalTuning immunoassay platform is a flow-based, bead-based multiplex system that enables high-precision intra-sample background correction at the individual analyte level.<\/p>\n\n\n\n

Traditional immunoassays like ELISA rely on buffer-only blank wells as negative controls. However, these fail to account for matrix effects\u2014nonspecific interactions from complex biological samples\u2014that often lead to false positives. Platforms such as ImmunoCAP use non-target\u2013coated sponges for background correction, but differences between the surface chemistry of target and non-target coatings often yield inconsistent and inaccurate background estimates.<\/p>\n\n\n\n

In flow cytometry, isotype controls are commonly used to assess nonspecific antibody binding, yet they rarely mimic the true background behavior of target-specific capture antibodies. Similarly, Luminex and Meso Scale Discovery (MSD) platforms implement intra-well background subtraction, typically using a single control bead to correct for nonspecific signal across all analytes in the same well. This \u201cone-control-for-all\u201d approach fails to address analyte-specific background variability, potentially compromising quantitative accuracy.<\/p>\n\n\n\n

The NOVEOS platform represents an improvement by incorporating dual bead types per well\u2014target-coated and non-coated control beads\u2014to correct for intra-sample background. However, NOVEOS data are collected on a per-well basis, limiting the statistical resolution and granularity of signal analysis.<\/p>\n\n\n\n

In contrast, the FocalTuning platform pairs each target capture bead with a matched control bead within the same sample. This design eliminates the need for buffer-only blanks, isotype antibodies, or external target-negative samples. Instead, it enables analyte-specific background subtraction using intra-sample, per-analyte controls. Because the platform analyzes data on a per-bead basis, it offers substantially greater statistical power and reproducibility than traditional per-well systems.<\/p>\n\n\n\n

In terms of sensitivity, the platform’s fluorescence-based limit of detection (LOD) reaches the low picogram-per-milliliter (pg\/mL) range. Furthermore, if metal isotope tags (as used in mass cytometry) are employed in place of fluorophores, the LOD can be further improved, potentially enabling sub-picogram or even femtogram-level detection.<\/p>\n\n\n\n

The FocalTuning platform supports both manual operation and automated, high-throughput processing, making it a versatile and scalable solution for both research and clinical diagnostics.<\/p>\n\n\n\n

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FocalTuning Assay Poster<\/a>Download<\/a><\/div>\n\n\n\n
Immunoassay Platform Comparison<\/a>Download<\/a><\/div>\n\n\n\n
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Type I hypersensitivity Diagnostics<\/a>Download<\/a><\/div>\n<\/div>\n<\/div>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

The FocalTuning immunoassay platform is a flow-based, bead-based multiplex system that enables high-precision intra-sample background correction at the individual analyte level. Traditional immunoassays like ELISA rely on buffer-only blank wells as negative controls. However, these fail to account for matrix effects\u2014nonspecific interactions from complex biological samples\u2014that often lead to false positives. Platforms such as ImmunoCAP … <\/p>\n

Continue reading “Why FocalTuning\u2122 a Superior Immunoassay Platform?”<\/span><\/a><\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-994","page","type-page","status-publish","hentry"],"jetpack-related-posts":[],"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/leadinglifetechnologies.com\/index.php?rest_route=\/wp\/v2\/pages\/994","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/leadinglifetechnologies.com\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/leadinglifetechnologies.com\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/leadinglifetechnologies.com\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/leadinglifetechnologies.com\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=994"}],"version-history":[{"count":28,"href":"https:\/\/leadinglifetechnologies.com\/index.php?rest_route=\/wp\/v2\/pages\/994\/revisions"}],"predecessor-version":[{"id":3291,"href":"https:\/\/leadinglifetechnologies.com\/index.php?rest_route=\/wp\/v2\/pages\/994\/revisions\/3291"}],"wp:attachment":[{"href":"https:\/\/leadinglifetechnologies.com\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=994"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}